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Eppendorf AG. (9/11/14). "Press Release: Next Automated NGS Library Preparation Method for the Eppendorf epMotion – TruSeq Stranded mRNA". Hamburg.

Organisations Organisation Eppendorf AG
  Group Eppendorf (Group)
  Organisation 2 Illumina Inc. (Nasdaq: ILMN)
  Group Illumina (Group)
Products Product epMotion workstation
  Product 2 TruSeq™ Sample Preparation Kit
Person Person Doerler, Bettina (Eppendorf 201403 Press Contact)
     


Today Eppendorf announces the availability of the epMotion automation method for Illumina's TruSeq Stranded mRNA library preparation kit for nextgeneration sequencing as "Illumina Qualified." "Illumina Qualified" indicates that Illumina's analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually.

For next generation sequencing systems a variety of sample preparation kits are available. These kits are needed to convert either DNA or RNA samples into sequencing ready libraries, a procedure that includes many steps and can be time consuming. RNA sequencing requires additional steps - either the depletion of unwanted ribosomal or the positive selection of mRNA from total RNA samples. Due to the complexity of the library construction methods, automation is regarded as highly useful.

The epMotion method can be used for the automated construction of 8, 16 or 24 libraries starting with 100 - 1000 ng of total RNA together with Illumina's TruSeq Stranded mRNA kit. The overall hands-on time is less than 1.5 hours, while the total run time of the entire procedure is ~11.5 hours for 24 samples. Minimized hands-on time & high reproducibility with this automated method help to streamline and standardize the sequencing
workflow.

Additional automated methods for NGS library preparation from Illumina are currently in development.


Press Contact:

Bettina Doerler
Barkhausenweg 1
22339 Hamburg, Germany
Phone: +49 40 538 01-640
Fax: +49 40 538 01-662
doerler.b@eppendorf.de
www.eppendorf.com


About Eppendorf AG:

Eppendorf is a leading life science company that develops and sells instruments, consumables, and services for liquid handling, sample handling, and cell handling in laboratories worldwide. Its product range includes pipettes and automated pipetting systems, dispensers, centrifuges, mixers, spectrometers, and DNA amplification equipment as well as ultra-low temperature freezers, fermentors, bioreactors, CO2 incubators, shakers, and cell manipulation systems. Consumables such as pipette tips, test tubes, microliter plates, and disposable bioreactors complement the range of highest-quality premium products.

Eppendorf products are most broadly used in academic and commercial research laboratories, e.g., in companies from the pharmaceutical and biotechnological as well as the chemical and food industries. They are also aimed at clinical and environmental analysis laboratories, forensics, and at industrial laboratories performing process analysis, production, and quality assurance.

Eppendorf was founded in Hamburg, Germany in 1945 and has about 2,850 employees worldwide. The company has subsidiaries in 25 countries and is represented in all other markets by distributors.

   
Record changed: 2017-04-02

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